2018 Summer Internship Program
Welcome to the Herman B Wells Center for Pediatric Research at the Indiana University School of Medicine and Riley Hospital for Children. In this package you will find all the details for the summer internship program. We have designed this program for you to get acquainted with Biomedical Research and some of the career paths that you can take in this field.
For the 9 weeks of the internship you will primarily be exposed to and conduct biomedical research in the laboratory you have been selected for. You will design and execute a research project in close consultation with your mentor. Students are expected to participate in research for at least 40 hours per week.
Before you start your research, the internship program will conduct laboratory safety training and you will complete a short test (see details below). Most laboratories hold their own specific weekly meetings, please check with your mentor regarding the location and times for these meetings.
Close to the end of the 9 weeks period you are expected to submit an Abstract describing the research you have conducted during the summer. A symposium will be held on August 1st and 2nd where you will give an oral presentation describing your summer project. Details for the format of the abstract and presentation are outlined below.
The internship program organizes several talks and discussion panels related to careers in research and other biomedical fields. A list for these talks is also included below. Lunch will be provided after each of these talks which will give you an opportunity to meet your fellow interns.
To successfully complete the summer internship you are required to attend all the events organized by the internship program (see list below). The interns will be awarded a certificate of completion once you have successfully completed the internship.
Should you have questions, please contact Lynn Pressler (firstname.lastname@example.org) or Laura Oxford (email@example.com). We hope you find your summer internship with us to be both a learning and rewarding experience.
Laboratory Safety Training
The laboratory safety training will be conducted on Monday, June 4th, 2018 along with the Welcome Lunch. Interns are required to complete a test on the Chemical Hygiene Program (CHP) and the Bloodborne Pathogen Program (BPP) and return it at the reception desk , R4 108 by June 11, 2018. To prepare for these tests visit the Environmental Health and Safety website at:
You will find the relevant information for the Chemical Hygiene Program or Bloodborne Pathogen Program on these websites respectively.
As a participant you are required to prepare an abstract describing your research project. A booklet containing abstracts from each of the participants will be prepared and distributed to you at the time of the Symposium.
Abstracts should be prepared on an 8 ½ x 11 size sheet of paper with 1 inch margins. Use only Arial font, size 11 and justify the paragraphs. Abstracts should include title, authors, institution affiliation information, and then the body of the abstract. The body of the abstract should be no more than 300 words. A typical scientific abstract contains the following information.
- A statement of the biological problem you are studying.
- Succinctly describing what is known in the area of your research.
- The specific question or hypothesis you are addressing in your project.
- The methodology you are using to address the question or hypothesis.
- The results and conclusions you have obtained with your research.
A sample abstract is included below for your reference. Please work with your faculty and/or lab mentor to prepare your abstract. It is your responsibility to email your abstract to Lynn Pressler (firstname.lastname@example.org) by 12:00 pm on Wednesay, July 25, 2018. Make sure that your name is on the file name.
You are required to present your summer research project in a Symposium organized by the internship program. The Symposium will be held on August 1st and 2nd (tentatively), 2018 from 9:00 am to 1:30 pm. Each participant will make an oral presentation for 10 minutes followed by a 5 minute question and answer session. The detailed schedule will be distributed closer to the date of the symposium. We encourage you to use PowerPoint to make your slides. For a 10 minute talk, typically 10-12 slides are sufficient. Make your slides and prepare your talk using the same guidelines as the abstract. We would like you to bring your PowerPoint presentation on a USB Drive on the day of your presentation. We will load your PowerPoint file on the computer connected to the projector before each session starts.
PARP-14 and the enzyme activity associated with it participate in the progression of allergic hyper-responsiveness
Purvi Mehrotra, Andrew Hollenbeck, Fang Li and Shreevrat Goenka
HB Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202
Interleukin-4 (IL-4) and Stat6 play an important role in progression of allergic airway hyper-responsiveness (AHR). In T cells IL-4 and Stat6 mediate a T helper 2 (Th2) response that promotes the asthmatic condition, and in B cells immunoglobulin class switching to IgE is dependent on IL-4 and Stat6. Previously, we have demonstrated that PARP-14, a member of the PARP family of proteins regulates the transcription function of Stat6. The ADP-ribosyl transferase enzyme activity associated with PARP-14 enhances the IL-4 dependent gene induction. Here we provide evidence that PARP-14 and the catalytic activity associated with it participate in the differentiation of T cells towards a Th2 phenotype. Moreover, using a mouse model of AHR we observe reduced lung pathology with PARP-14 deficiency as compared to control animals. The antigen specific IgE is also significantly reduced in mice lacking PARP-14 expression. Further, treating mice with a pharmacological inhibitor for ADP-ribosylation reduces the severity of antigen induced AHR. Taken together, these data indicate that PARP-14 and its catalytic activity participate in allergic AHR, and targeting PARP-14 may be a potential new therapy for chronic asthma.