Edward F. Srour, PhD Professor: Department of Medicine and Pediatrics
Professor: Department of Medicine and Pediatrics
Director: Flow Cytometry Resource Facility
Basic Science Joint Appointment: Microbiology and Immunology
Postdoctoral Fellowship: Indiana University School of Medicine, Indianapolis, IN
PhD: University of Illinois, Urbana-Champaign, IL, 1986
Current Research Interests:
Hematopoietic stem cell activation, proliferation, differentiation and homing. Transdifferentiation potential of pluripotent stem cells.
My research program has focused on the characterization and biology of human hematopoietic stem cells and their use in bone marrow transplantation and as efficient vehicles for retroviral mediated somatic gene transfer. The laboratory is also interested in examining the specificity and mechanisms involved in directed homing of stem cells to the bone marrow following transplantation and during ontogeny. In the last few years we began investigating the pluripotential transdifferentiation capacity of other stem cells present in different mammalian tissues.
Ongoing projects include:
- Examination of the relationship between cell cycle status of primitive hematopoietic progenitor cells and its effect on engraftment and maintenance of bone marrow repopulating potential. An interesting recent finding in our laboratory is that mitotic quiescence, a hallmark of hematopoietic function of stem cells from adult tissues, is not required among similar fetal cells or those isolated from umbilical cord blood. The nature of these differences between pre-and neonatal sources of stem cells and adult tissues and how it may impact the utility of these cells in clinical transplantation and gene therapy is now the focus of research in one of the projects in the laboratory.
- Involvement of adhesion molecules and homing receptors in trafficking and migration of hematopoietic stem cells between different hematopoietic sites during ontogeny and in directing homing of transplanted stem cells to the bone marrow are investigated both in vitro and in vitro. Also relevant changes in expression of these molecules in relation to cell cycle progression and bone marrow repopulating potential are examined. Finally in these series of studies, the behavior of stem cells immediately after homing to the bone marrow is examined to determine how that impacts the long-term engraftment potential of these cells.
- In the third area of research, the transdifferentiation capacity of pluripotent stem cells isolated from different mammalian tissues is evaluated. We recently identified a unique phenotype of pluripotent stem cells that have been detected in 6 different murine tissues. Cells from all sites remarkably differentiated into hematopoietic cells when transplanted into appropriately conditioned recipients. The exact nature, origin and the full potential of these cells, along with determination of whether these cells are capable of clonal transdifferentiation into multiple tissue lineages are now the subject of intense investigations in the laboratory.